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Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvemen… Mehr…

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Heterologous Gene Expression in E.coli: Methods and Protocols: Vol 705 - gebunden oder broschiert

2010, ISBN: 1617379662

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Evans, Thomas C., Jr. (Editor):
Heterologous Gene Expression in E.coli: Methods and Protocols: Vol 705 - gebunden oder broschiert

2010

ISBN: 9781617379666

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Jr. Evans, Thomas C.; Ming-Qun Xu:
Heterologous Gene Expression in E.coli - gebunden oder broschiert

2010, ISBN: 9781617379666

Methods and Protocols, Buch, Hardcover, 2011, [PU: Humana Press Inc.], Humana Press Inc., 2010

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Details zum Buch
Heterologous Gene Expression in E.coli
Autor:
Titel:
ISBN-Nummer:

9781617379666


Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli. In Heterologous Gene Expression in E. coli: Methods and Protocols, expert scientists intimately familiar with the relevant techniques offer chapters that greatly expand the utility of this expression host. The contributions in this detailed volume describe methods, for example, to successfully express proteins in E. coli that would otherwise form aggregates in this host, to add post-translational modifications, to incorporate non-standard amino acid residues or moieties into E. coli expressed proteins, to identify binding partners, and to express membrane proteins. Written in the highly successful Methods in Molecular Biology(TM) format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Heterologous Gene Expression in E. coli: Methods and Protocols seeks to familiarize the researcher with the myriad of E. coli expression strains available and move E. coli closer to that ideal of the perfect host.

Detailangaben zum Buch - Heterologous Gene Expression in E.coli


EAN (ISBN-13): 9781617379666
ISBN (ISBN-10): 1617379662
Gebundene Ausgabe
Erscheinungsjahr: 2011
Herausgeber: Humana Press Inc.
307 Seiten
Gewicht: 0,758 kg
Sprache: eng/Englisch

Buch in der Datenbank seit 2007-10-23T23:28:59+02:00 (Berlin)
Detailseite zuletzt geändert am 2023-07-15T15:04:15+02:00 (Berlin)
ISBN/EAN: 9781617379666

ISBN - alternative Schreibweisen:
1-61737-966-2, 978-1-61737-966-6
Alternative Schreibweisen und verwandte Suchbegriffe:
Autor des Buches: ming, thomas christopher, thomas evans, qun, chris thomas
Titel des Buches: expression, ming, qun

Daten vom Verlag:

Autor/in: Thomas C. Evans, Jr.; Ming-Qun Xu
Titel: Methods in Molecular Biology; Heterologous Gene Expression in E.coli - Methods and Protocols
Verlag: Humana; Humana Press
310 Seiten
Erscheinungsjahr: 2010-12-02
Totowa; NJ; US
Gedruckt / Hergestellt in Niederlande.
Sprache: Englisch
160,49 € (DE)
164,99 € (AT)
177,00 CHF (CH)
POD
XI, 310 p.

BB; Hardcover, Softcover / Medizin/Klinische Fächer; Genetik, Medizin; Verstehen; Amino acid residues or moieties; Binding partners; Escherichia coli; Expression hosts; Membrane proteins; Post-translational modifications; Protein expression; Recombinant DNA; Recombinant proteins; Proteomics; Medical Genetics; Protein Biochemistry; Biochemie; EA; BC

Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli. In Heterologous Gene Expression in E. coli: Methods and Protocols, expert scientists intimately familiar with the relevant techniques offer chapters that greatly expand the utility of this expression host. The contributions in this detailed volume describe methods, for example, to successfully express proteins in E. coli that would otherwise form aggregates in this host, to add post-translational modifications, to incorporate non-standard amino acid residues or moieties into E. coli expressed proteins, to identify binding partners, and to express membrane proteins. Written in the highly successful Methods in Molecular Biology™ format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Heterologous Gene Expression in E. coli: Methods and Protocols seeks to familiarize the researcher with the myriad of E. coli expression strains available and move E. coli closer to that ideal of the perfect host.
Helps to guide scientists toward an ideal expression host Provides easy to follow protocols, ready to be implemented in the lab Includes tips from the experienced, expert contributors on troubleshooting common methodological pitfalls Includes supplementary material: sn.pub/extras
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