Advanced Technologies for Protein Complex Production and Characterization - neues Buch

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ISBN/EAN: 9783319272160

ISBN - alternative Schreibweisen:
978-3-319-27216-0
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Autor des Buches: vega

Daten vom Verlag:

Autor/in: M. Cristina Vega
Titel: Advances in Experimental Medicine and Biology; Advanced Technologies for Protein Complex Production and Characterization
Verlag: Springer; Springer International Publishing
382 Seiten
Erscheinungsjahr: 2016-05-10
Cham; CH
Sprache: Englisch
181,89 € (DE)
187,00 € (AT)
212,50 CHF (CH)
Available
XIII, 382 p. 87 illus., 71 illus. in color.

EA; E107; eBook; Nonbooks, PBS / Biologie/Biochemie, Biophysik; Biochemie; Verstehen; Acid complexes; Protein complexes; Expression systems; Eukaryotic expression systems; Structural and biophysical characterization; Protein Structure; Systems Biology; B; Protein Biochemistry; Proteins; Computational and Systems Biology; Biomedical and Life Sciences; DV-gestützte Biologie/Bioinformatik; BB

), baculovirus-infected insect cells, mammalian cells, plants and algae. Complex reconstitution from individually purified subunits or subcomplexes is discussed as a complementary strategy. A last section introduces briefly some of the biophysical and structural characterization techniques for macromolecular complexes using state-of-the-art solution scattering and nuclear magnetic resonance.

This work is a guided tour over some of the most powerful and successful protein expression technologies, with a focus on co-expression and high-throughput applications. It is addressed to everyone interested in the production and characterization of macromolecular complexes, from university students who want an accessible description of the major co-expression systems to researchers in biomedicine and the life sciences seeking for an up-to-date survey of available technologies.

Protein complex production from the drug discovery standpoint.- Choose a suitable expression host: a survey of available protein production platforms.- ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts.- Complex reconstitution and characterization by combining co-expression techniques in Escherichia coli with high-throughput.- Membrane protein production in E. coli for applications in drug discovery.- Cell-free Synthesis of Macromolecular Complexes.- A Bacillus megaterium system for the production of recombinant proteins and protein complexes.- Protein complex production in alternative prokaryotic hosts.- Production of Protein Complexes in Non-methylotrophic and Methylotrophic Yeasts.- Leishmania tarentolae for the Production of Multi-Subunit Complexes.- Alternative Eukaryotic Expression Systems for the Production of Proteins and Protein Complexes.- Fundamentals of baculovirus expression and applications.- The MultiBac Baculovirus / Insect Cell Expression Vector System for Producing Complex Protein Biologics.- Fundamentals of expression in mammalian cells.- Assembling multi-subunit complexes using mammalian expression.- Microalgae as solar-powered protein factories.- Strategies and methodologies for the co-expression of multiple proteins in plants.- Transient expression systems in plants—potentialities and constraints.- Complex reconstitution from individual protein modules.- Structural reconstruction of protein-protein complexes involved in intracellular signaling.- The use of small-angle scattering for the characterization of multi subunit complexes.- Application of nuclear magnetic resonance and hybrid methods to structure determination of complex systems.   

is a group leader at the Chemical and Physical Biology Department of the Center for Biological Research (CIB-CSIC) in Madrid, Spain. With substantial experience in macromolecular X-ray crystallography of protein complexes with biomedical relevance, she designs eukaryotic, yeast-based expression platforms tailored to protein complexes with a focus on cost-efficiency and improved performance. 

, yeast, mammalian and insect cells to complex reconstitution from individual subunits, this book offers useful insights and guidance for successful protein expressionists.

), baculovirus-infected insect cells, mammalian cells, plants and algae. Complex reconstitution from individually purified subunits or subcomplexes is discussed as a complementary strategy. A last section introduces briefly some of the biophysical and structural characterization techniques for macromolecular complexes using state-of-the-art solution scattering and nuclear magnetic resonance.

This work is a guided tour over some of the most powerful and successful protein expression technologies, with a focus on co-expression and high-throughput applications. It is addressed to everyone interested in the production and characterization of macromolecular complexes, from university students who want an accessible description of the major co-expression systems to researchers in biomedicine and the life sciences seeking for an up-to-date survey of available technologies.