Advanced Protocols for Animal Transgenesis: An ISTT Manual Shirley Pease Editor - neues Buch

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ISBN/EAN: 9783642207921

ISBN - alternative Schreibweisen:
978-3-642-20792-1
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Autor des Buches: saunders, pease, thomas
Titel des Buches: anima, anim

Daten vom Verlag:

Autor/in: Shirley Pease; Thomas L. Saunders
Titel: Springer Protocols Handbooks; Advanced Protocols for Animal Transgenesis - An ISTT Manual
Verlag: Springer; Springer Berlin
669 Seiten
Erscheinungsjahr: 2011-08-30
Berlin; Heidelberg; DE
Sprache: Englisch
149,79 € (DE)
154,00 € (AT)
177,00 CHF (CH)
Available
XV, 669 p. 210 illus., 65 illus. in color.

EA; E107; eBook; Nonbooks, PBS / Biologie/Genetik, Gentechnik; Genetik (nicht-medizinisch); Verstehen; Embryonic stem cells; Knocked out genes; Nuclear gene transfer; Pluripotent cells; Transgenic animals; Transposable elements; YACs; Transgenic organisms; B; Genetics and Genomics; Experimental Organisms; Biomedical and Life Sciences; Biologie, Biowissenschaften; Wissenschaftliche Ausstattung, Experimente und Techniken; BB

This laboratory manual, published in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections. Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.

Teruhiko Wakayama: Mouse Cloning by Nuclear Transfer.- Elizabeth D. Hughes and Thomas L. Saunders: Gene Targeting in Embryonic Stem Cells.- Wojtek Auerbach and Anna B. Auerbach: The Importance of Mouse ES Cell Line Selection.- Marina Gertsenstein: Tetraploid Complementation Assay.- Elizabeth Williams et al.: Combining ES cells with Embryos.- Kristina Nagy and Jennifer Nichols: Derivation of Murine ES Cell Lines.- Ping Li et al.: Rat Embryonic Stem Cell Derivation and Propagation.- Han Li et al.: Induced pluripotency: generation of iPS cells from mouse embryonic fibroblasts.- Anna B. Auerbach, Peter J. Romanienko, and Willie H. Mark: The Preparation and Analysis of DNA for Use in Transgenic Technology.- Karen Brennan: Colony Management.- B. Pintado and J Hourcade: Cryopreservation.- ShirleyPease: Shipment of Mice and Embryos.- J.M. Sztein, R.J. Kastenmayer, K.A. Perdue: Pathogen Free Mouse Rederivation by IVF, Natural Mating and Hysterectomy.- Jan Parker-Thornburg: Refinement, Reduction and Replacement.

This laboratory manual, published in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections.

Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.